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DOI: 10.1038/ncomms4930

Divergence in AS variants of gene pairs presents an important layer of gene regulation, as reported35–38, and thus provides a genetic basis for species evolution and new species formation.

This subfunctionalization or neofunctionalization of duplicated genes provides genetic novelty and a basis for species evolution and new species formation

Gene expression variation may contribute to an increased complexity of regulatory networks after polyploidization.

https://www.ncbi.nlm.nih.gov/pubmed/19858364

Alternative splicing can enhance transcriptome plasticity and proteome diversity.

Estimates have suggested that ∼22%–30% of Arabidopsis intron-containing genes are alternatively spliced

We predict that at least ∼42% of intron-containing Arabidopsis genes are alternatively spliced. This estimate is significantly higher than previous estimates based on cDNA/EST sequencing.

Extensive Divergence in Alternative Splicing Patterns after Gene and Genome Duplication During the Evolutionary History of Arabidopsis

Genome duplication (polyploidy) has been a common phenomenon among eukaryotes of various groups, including plants, animals, and some single-celled eukaryotes.

Despite the importance of AS to gene expression, the conservation of AS patterns after WG duplication remains unknown, and AS events for a sizable set of duplicated gene pairs have not been examined and compared in plants

By comparing each subgenome in tetraploids with its extant diploid progenitor

Identification and analysis of alternative splicing events in Phaseolus vulgaris and Glycine max

Animals tend to have very large introns and therefore the splicing machinery recognizes exons (exon definition) while in plants the spliceosome recognizes introns (intron definition) [28].